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Tryptone 35 Yeast Extract 20 NaCl 5 Agar 15 Final pH 7
M9 is a chemical defined minimal medium supporting the growth of "wild-type" strains of E
2 Glucose 2 KH2PO4 1
5x is a 5x concentrate and has to be diluted to a 1x concentration and subsequently supplemented with an appropriate carbon source
LB-Top Agar Miller Size:250g Tryptone 35 Yeast Extract 20Formula g l Tryptone 10 Yeast extract 5 NaCl 10 Agar 7 Final pH 7. 0 0. 2 at 25C Suspend 32 gram powdered medium in 1 litre distilled water Store dry at room temperature
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